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Updated: Jun 29, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
Published on: March 7, 2014
The use of 2-D gels to identify novel protein-protein interactions in the cochlea
Thandavarayan Kathiresan1, Margaret C Harvey, Bernd H A Sokolowski
1Department of Otolaryngology-HNS, University of South Florida College of Medicine, Tampa, FL, USA.
Abstract:
Functional proteomics comprises a wide range of technologies for the identification of novel protein-protein interactions and biological markers. Studies of protein-protein interactions have gained from the development of techniques and technologies such as immunoprecipitation, preparative two-dimensional (2-D) gel electrophoresis for peptide mass fingerprinting (PMF), using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). These applications enabled the discovery of putative protein partners without a priori knowledge of which one(s) might be relevant. Here, we report the methods by which membrane proteins are isolated from cochlear tissues and prepared for identification by mass spectrometry techniques.
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