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Updated: Jun 29, 2026

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
Published on: November 3, 2017
Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate
Kenji Nagao1, Takayuki Ohta, Atsushi Hinohara
1Frontier Laboratory, Kirin Pharma CO, LTD, Takasaki, Gunma, Japan.
Abstract:
The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-S3, one of which was hematopoiesis supportive (S3-A9) and the other one of which was non-supportive (S3-A7), and we analyzed their gene expression profiles by gene chip analysis. In the present study, we found that Glypican-1 (GPC1) was highly expressed in the supportive subclone AGM-S3-A9. Over-expression of GPC1 in non-supportive cells led to the proliferation of progenitor cells in human cord blood when cocultured with the transfected-stromal cells. Thus, GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis.
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