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Updated: Jun 29, 2026
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
Published on: September 10, 2021
SSCP analysis of scnDNA for genetic profiling of Aedes aegypti
Jacklyn Wong1, Frédéric Tripet, Jason L Rasgon
1Department of Entomology, University of California, Davis, California 95616, USA. jacwong@ucdavis.edu
Abstract:
We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.
