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[Search for Fanconi anemia/BRCA pathway defects in lymphoma cell lines]
Hui Xiao1, Kejian Zhang, Bing Xia
1Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China. huixiaowh@yahoo.com.cn
Objective:
To investigate the possible relationship between defects in the FA/BRCA pathway of genomic stability and potential pathogenesis of T and B cell lymphoma.
Methods:
Nineteen cell lines derived from diverse subtypes of lymphoma for possible FA pathway defects were screened.
Results:
No defect in FANCD2 ubiquitination was observed. However, the FANCN protein was absent in cell lines HT and Sudhl4. This absence was correlated with enhanced MMC-induced G2 arrest, growth inhibition and high chromosomal breakage rate in both cell lines. In addition, in exon-5a of FANCN gene, a mutation of c.1769 C>T, p. A590V was found in cell line HT, but not in cell line Sudhl4.
Conclusion:
This mutation may be the reason causing the absence of the FANCN protein expression or making the protein unstable and losing its function.
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