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Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

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Published on: November 10, 2021

Encapsulation efficiency measured on single small unilamellar vesicles.

Brian Lohse¹, Pierre-Yves Bolinger, Dimitrios Stamou

¹Bio-Nanotechnology Laboratory, Department of Neuroscience and Pharmacology & Nano-Science Center, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark.

Journal of the American Chemical Society

|October 10, 2008

Summary

This study introduces a fluorescent method to measure encapsulation efficiency in single small unilamellar vesicles (SUVs). Researchers found encapsulation efficiency decreases with vesicle radius, with many SUVs being empty, insights lost in bulk measurements.

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Construction of Out-of-Equilibrium Metabolic Networks in Nano- and Micrometer-Sized Vesicles

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Published on: April 12, 2024

Area of Science:

Biophysics
Materials Science
Analytical Chemistry

Background:

Accurate measurement of encapsulation efficiency is crucial for drug delivery systems.
Bulk measurement methods can obscure critical information about individual vesicle performance.
Small unilamellar vesicles (SUVs) are widely used nanocarriers.

Purpose of the Study:

To develop and validate a fluorescence-based method for quantifying encapsulation efficiency in single SUVs.
To investigate the relationship between SUV size and encapsulation efficiency.
To identify potential limitations of bulk measurement techniques for SUVs.

Main Methods:

Loading SUVs with membrane and lumen dyes.
Immobilizing single SUVs on a surface for imaging.
Utilizing fluorescence microscopy to determine vesicle size and lumen content.
Correlating membrane and lumen dye signals to calculate encapsulation efficiency.

Main Results:

Developed a single-vesicle assay for encapsulation efficiency.
Demonstrated encapsulation efficiency is inversely proportional to vesicle radius.
Observed a significant population of empty vesicles, not detectable by bulk methods.
Findings validated for vesicles prepared via the rehydration technique.

Conclusions:

The developed method provides high-resolution insights into SUV encapsulation.
Vesicle size significantly impacts encapsulation efficiency, challenging uniform drug loading assumptions.
Single-vesicle analysis is essential for a comprehensive understanding of SUV-based formulations and can reveal limitations of bulk analysis.