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Protein kinase C activity and intracellular distribution in surgically excised human epileptic neocortex
O Vernet1, V W Yong, K Rostworowski
1Montreal Neurological Institute, McGill University, Qué, Canada.
Brain Research
|May 3, 1991
Abstract:
Protein kinase C (PKC) activity assayed by phosphorylation of exogenous histone, was measured in neocortex obtained from 32 patients following surgery for focal epilepsy and from 6 non-epileptic patients. PKC activity was not significantly different in either the particulate or cytosolic fraction from epileptic foci (n = 17) versus samples from non-spiking regions (n = 22) or neocortex from non-epileptic patients (n = 6). From 67% to 70% of total PKC activity was present in the cytosolic fraction. Phosphorylation of endogenous cytosolic substrate proteins was also not significantly different in epileptic foci.