Chemotaxis analysis of circulating monocytes in patients with a recent acute coronary syndrome

Monocytes/macrophages are crucially involved in the process of atherogenesis. Presence of an acute coronary syndrome (ACS) is associated with macrophage activation. We investigated whether ligand-induced monocyte chemotaxis can serve as biomarker in recent ACS and discriminate ACS from stable coronary artery disease (CAD).

In a prospective study, the migratory response of monocytes towards the chemotactic ligands vascular endothelial growth factor-A (VEGF-A) and monocyte chemoattractant protein-1 (MCP-1) in patients with recent ACS (n=29) (median time period since cardiovascular event, 11 days) and stable CAD patients (n=41) was analysed. Furthermore, blood levels of C-reactive protein (CRP), VEGF-A and soluble vascular endothelial growth factor receptor-1 (sVEGRF-1) were determined.

Unexpectedly, VEGF-A-induced monocyte chemotaxis did not differ between ACS and CAD. The same was true for the chemotactic response of monocytes towards MCP-1. In addition, we could not find any difference in VEGF-A and sVEGFR-1 levels between recent ACS and stable CAD. CRP was significantly enhanced in the ACS group, but did not correlate with the VEGF-A- and MCP-1-induced chemotaxis.

Recent ACS is not associated with enhanced monocyte chemotaxis towards VEGF-A and MCP-1. Therefore, VEGF-A- and MCP-1-induced monocyte chemotaxis as a potential novel biomarker remains unaffected by recent ACS.