Related Experiment Video
Updated: Jun 29, 2026

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Published on: November 29, 2018
Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen
B Macías García1, J M Morrell, C Ortega-Ferrusola
1Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avda de Universidad s/n, Cáceres, Spain.
Abstract:
The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E). Sperm motility, sperm chromatin structure, membrane integrity and mitochondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI.
More Related Videos
09:16U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
Published on: March 28, 2025
06:40Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
Published on: October 24, 2025
Related Concept Videos
Centrifugation
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...