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Updated: Jun 29, 2026
Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
Published on: December 2, 2022
[Expression of bcl-2, Ki-67 and p-53 in uveal melanomas]
Ana Krvavica1, Jasna Talan-Hranilović, Mladen Belicza
1Odjel patologije i sudske medicine, Opća bolnica, Zadar. ana.krvavica@zd.htnet.hr
Aim:
To investigate expression of proliferative markers bcl-2, Ki-67 and p-53 immunocomplexes in uveal melanoma cells; and to establish whether the intensity of expression correlates with the pathological level of invasion (pT), which would result in prognostic significance.
Methods:
Thirty cases of primary uveal melanomas of two different levels of invasion (pT2 and pT3); indirect PAP immunoenzyme method and three step ABC/AP method. The intensity of the reaction was evaluated by a semiquantitative method as negative (-), weakly positive (+), modestly positive (++) and strongly positive (+++). Results were statistically analyzed by Fisher exact test for small examples.
Results:
The reactivity of Ki-67 and p-53 protein was markedly stronger than bcl-2 in pT2 and pT3 stages. p-53 protein expression showed similar distribution for Ki-67 protein according to pT stages.
Conclusion:
The reactivity of all three markers was stronger in pT3 stage, especially of proliferation markers Ki-67 and p-53. In addition to the fact that they both are reliable indicators of the metastatic potential, Ki-67 is a better marker because there is a well established technique for measuring cell proliferation in all phases of the cell cycle.
