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Updated: Jun 29, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
[Overexpression of core binding factor alpha1 enhancing osteoblastic differentiation in adipose-derived stem cells]
1Institute of Sports Medicine, the Third China Hospital of Peking University, Beijing 100083, China.
Objective:
To determine whether mouse core binding factor alpha1 (Cbfalpha1) overexpression enhancing osteoblastic differentiation in adipose-derived stem cells (ADSCs).
Methods:
ADSCs were harvested from SD rats and transduced with the recombinant adenovirus carrying Cbfalpha1 gene. Untransduced cells and cells transduced with adenovirus carrying the enhanced green fluorescence protein (Ad-EGFP) gene served as controls. Cbfalpha1 expression was assessed by immunofluorescence. Alkaline phosphatase (ALP) activity was assayed in 7 and 10 d.
Results:
Overexpression of Runx2 inhibited adipogenesis, as demonstrated by suppression of LPL expression. Moreover, ADSCs transduced with Ad-Runx2 underwent rapid and marked osteoblast differentiation as determined by osteoblastic gene expression, alkaline phosphatase activity.
Conclusions:
Cbfalpha1 overexpression enhances osteoblastic differentiation in adipose-derived stem cells.
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