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Published on: February 6, 2015
[Effect of cisplatin on the expression of Pokemon gene: experiment with different human lung cancer cells]
Zhi-Hong Zhao1, Sheng-Fa Wang, Liang Yu
1Department of Chest Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. zzh517cs@yahoo.com.cn
Objective:
To investigate the correlation between Pokemon gene and cisplatin mechanism.
Methods:
Human lung adenocarcinoma cells of the lines A549 and AGZY83-a, human lung squamous carcinoma cells of the line HE-99, and human giant cell lung cancer cells of the line 95D were cultured and cisplatin was added into the medium. Other lung cancer cells of the above mentioned lines were cultured in the medium without cisplatin and were used as control groups. RT-PCR and Western blotting were used to detect the mRNA and protein expression of Pokemon.
Results:
Pokemon mRNA and protein were expressed highly in all the 4 cell lines. The Pokemon gene expression did not changed significantly after cisplatin treatment groups. There were not significant differences in the mRNA and protein expression of Pokemon among the 4 experiment groups and the control groups (all P > 0.05).
Conclusion:
Cisplatin has no effect on the Pokemon gene expression of the human lung cancer cells.
