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Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

Atomic Absorption Spectroscopy: Overview

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...

Atomic Absorption Spectroscopy: Interference

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...

Atomic Absorption Spectroscopy: Instrumentation

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...

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Related Experiment Video

Updated: Jun 29, 2026

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

Published on: November 9, 2015

[Study on lead absorption in pumpkin by atomic absorption spectrophotometry].

Zhen-Xia Li¹, Yong-Dong Sun, Bi-Hua Chen

¹College of Landscape, Henan Institute of Science and Technology, Xinxiang 453003, China. lizhenxia196@hist.edu.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|October 11, 2008

Summary

This study shows lead absorption in pumpkins increases over time but at a slower rate. Optimal lead absorption in pumpkins occurs at pH 7, similar to cadmium absorption.

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Area of Science:

Environmental Science
Plant Biology
Toxicology

Context:

Lead contamination poses risks to food safety and agricultural sustainability.
Understanding heavy metal uptake in edible plants is crucial for risk assessment.

Purpose:

To investigate the kinetics and optimal conditions for lead absorption in pumpkin plants.
To compare lead and cadmium absorption characteristics in pumpkins.

Summary:

Lead absorption in pumpkin (Cucurbita pepo) increased with exposure time, while the absorption rate diminished over time.
The maximum lead absorption was observed at a neutral pH (pH 7).
Pumpkin exhibited similar absorption patterns for both lead and cadmium.

Impact:

Provides essential data for managing lead contamination in pumpkin cultivation.
Informs strategies for mitigating heavy metal accumulation in food crops.
Contributes to understanding plant-metal interactions for improved agricultural practices.