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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

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Published on: July 21, 2018

Fluorescence quantification for surface plasmon excitation.

Bob E Feller1, James T Kellis, Luis G Cascão-Pereira

  • 1Department of Chemical Engineering, Stanford University, Stanford, CA 94305-5025, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|October 11, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method to accurately measure surface processes using combined spectroscopy. The technique corrects for intensity variations, enabling simultaneous monitoring of protein adsorption and degradation.

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Area of Science:

  • Biophysical Chemistry
  • Surface Science
  • Spectroscopy

Background:

  • Surface plasmon resonance (SPR) and surface plasmon fluorescence spectroscopy (SPFS) are powerful tools for studying surface interactions.
  • Nonlinear responses in fluorescence intensity due to surface intensity variations pose challenges in multicomponent surface process analysis.

Purpose of the Study:

  • To develop a method to correct for surface intensity variations in combined SPR and SPFS.
  • To enable simultaneous monitoring of protease adsorption and proteolytic substrate degradation.

Main Methods:

  • Utilized the experimentally measured relationship between fluorescence and reflectivity to account for surface intensity variations.
  • Employed a layer-by-layer technique to prepare multilayer protein substrates for degradation studies.

Main Results:

  • Successfully developed and applied a method to correct for nonlinear fluorescence responses caused by surface intensity variations.
  • Demonstrated the simultaneous monitoring of protease adsorption and substrate degradation using the developed method.

Conclusions:

  • The developed method enhances the capability of combined SPR and SPFS for analyzing complex surface processes.
  • This approach provides a robust way to study enzyme kinetics and surface interactions in real-time.