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Updated: Jun 29, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Transcriptional activation of Cidec by PPARgamma2 in adipocyte

Yoon-Jin Kim1, Si Young Cho, Cheol Hee Yun

  • 1Department of Biology, Kyung Hee University, Seoul, Republic of Korea.

Biochemical and Biophysical Research Communications
|October 11, 2008
PubMed

Insights

Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) transcriptionally regulates Cidec, a protein that promotes triglyceride accumulation in adipocytes. This finding clarifies a key mechanism in lipid droplet formation during adipogenesis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Metabolism

Background:

  • Cidec (Cell death-inducing DFF45-like effector C) is a lipid droplet-associated protein inhibiting lipolysis.
  • Cidec promotes triglyceride accumulation in adipocytes, but its transcriptional regulation in this cell type is unclear.
  • Understanding Cidec regulation is crucial for elucidating lipid droplet formation mechanisms.

Purpose of the Study:

  • To investigate the transcriptional regulation of the mouse Cidec gene in adipocytes.
  • To determine the role of Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) in regulating Cidec expression during adipogenesis.

Main Methods:

  • Analysis of Cidec and PPARγ2 expression patterns during adipocyte differentiation.
  • Treatment with a PPARγ agonist to assess effects on Cidec mRNA levels.
  • Identification of Peroxisome proliferator response elements (PPREs) in the Cidec promoter.
  • Chromatin immunoprecipitation (ChIP) and reporter assays to confirm PPARγ2 binding to the Cidec promoter.
  • Gel shift and mutagenesis assays to pinpoint the functional PPRE region (-219/-207) in the Cidec promoter.

Main Results:

  • Cidec expression pattern mirrors PPARγ2 expression during adipocyte differentiation.
  • PPARγ agonist treatment significantly increases Cidec mRNA levels.
  • PPARγ2 directly binds to the Cidec promoter, specifically to the -219/-207 region, which acts as a functional PPRE.

Conclusions:

  • PPARγ2 is a key regulator of Cidec transcriptional activity during adipogenesis.
  • This regulatory relationship contributes to understanding the molecular mechanisms underlying lipid droplet formation in adipocytes.

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