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Related Concept Videos

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

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Related Experiment Video

Updated: Jun 29, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Cdk-counteracting phosphatases unlock mitotic exit.

Ethel Queralt1, Frank Uhlmann

  • 1Cancer Epigenetics and Biology Program (PEBC), Catalan Institute of Oncology, Av. Gran Via S/N, 08907 L'Hospitalet de Llobregat, Barcelona, Spain. equeralt@iconcologia.net

Current Opinion in Cell Biology
|October 11, 2008
PubMed
Summary

Mitotic exit requires both cyclin-dependent kinase (Cdk) downregulation and phosphatase activity to reverse phosphorylation. In budding yeast, Cdc14 phosphatase is key, with growing evidence for similar roles in metazoans.

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Last Updated: Jun 29, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

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Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Mitotic entry is regulated by cyclin-dependent kinase (Cdk) activity.
  • Mitotic exit requires Cdk activity decline and reversal of phosphorylation events.
  • The Cdc14 phosphatase is crucial for mitotic exit in budding yeast.

Purpose of the Study:

  • To investigate the role of protein dephosphorylation in mitotic exit.
  • To understand the regulation and function of phosphatases in cell cycle progression.
  • To explore the conservation of phosphatase-mediated mitotic exit in metazoan cells.

Main Methods:

  • Cell cycle analysis in budding yeast.
  • Biochemical assays to study phosphatase activity.
  • Investigating phosphatase roles in metazoan cell models.

Main Results:

  • Cdc14 phosphatase orchestrates ordered mitotic progression in budding yeast.
  • Cdk downregulation alone is insufficient for mitotic exit.
  • Evidence suggests conserved roles for regulated phosphatases in metazoan mitotic exit.

Conclusions:

  • Regulated protein dephosphorylation by phosphatases is essential for timely mitotic exit.
  • The mechanisms governing mitotic exit are conserved across eukaryotes, with phosphatases playing critical roles.
  • Further research is needed to fully elucidate phosphatase function in metazoan cell cycle regulation.