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Related Experiment Video

Updated: Jun 29, 2026

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

CTLA-4 control over Foxp3+ regulatory T cell function.

Kajsa Wing¹, Yasushi Onishi, Paz Prieto-Martin

¹Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

Science (New York, N.Y.)

|October 11, 2008

Summary

Cytotoxic T lymphocyte antigen 4 (CTLA-4) is crucial for regulatory T cell (Treg) function. Its deficiency in Tregs causes autoimmune disease and enhances anti-tumor immunity by impacting antigen-presenting cells.

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Last Updated: Jun 29, 2026

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Area of Science:

Immunology
Cell Biology
Autoimmunity

Background:

Foxp3+CD4+ regulatory T cells (Tregs) are vital for immune tolerance and homeostasis.
Cytotoxic T lymphocyte antigen 4 (CTLA-4) plays a role in T cell regulation.

Purpose of the Study:

To investigate the function of CTLA-4 specifically within Tregs.
To determine the consequences of Treg-specific CTLA-4 deficiency on immune responses.

Main Methods:

Utilized a mouse model with a specific deficiency of CTLA-4 in Tregs.
Assessed Treg suppressive function in vitro and in vivo.
Analyzed immune cell populations, autoantibodies, and tumor immunity.

Main Results:

Treg-specific CTLA-4 deficiency led to lymphoproliferation and fatal autoimmune disease in mice.
This deficiency also resulted in potent anti-tumor immunity.
Impaired Treg suppressive function was observed, particularly in down-regulating CD80/CD86 on dendritic cells.

Conclusions:

CTLA-4 is essential for the suppressive function of natural Tregs.
Tregs require CTLA-4 to modulate antigen-presenting cell potency and prevent autoimmunity.
Targeting CTLA-4 in Tregs may offer therapeutic strategies for autoimmune diseases and cancer immunotherapy.