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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Related Experiment Video

Updated: Jun 29, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Next-generation DNA sequencing.

Jay Shendure1, Hanlee Ji

  • 1Department of Genome Sciences, University of Washington, Seattle, Washington 98195-5065, USA. shendure@u.washington.edu

Nature Biotechnology
|October 11, 2008
PubMed
Summary
This summary is machine-generated.

Next-generation DNA sequencing offers powerful, cost-effective tools for biological research. These advancements democratize high-throughput data collection, accelerating discoveries in genomics and beyond.

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Nanopore DNA Sequencing for Metagenomic Soil Analysis

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Related Experiment Videos

Last Updated: Jun 29, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology
  • Bioinformatics

Background:

  • DNA sequencing is a foundational technology for biological research.
  • Recent advancements in massively parallel sequencing have drastically reduced costs.
  • This has made high-throughput DNA sequencing accessible to individual researchers.

Purpose of the Study:

  • To highlight the impact of next-generation DNA sequencing technologies.
  • To discuss the challenges and opportunities presented by these new platforms.
  • To emphasize the potential for accelerating biological and biomedical research.

Main Methods:

  • Massively parallel DNA sequencing platforms.
  • Development of robust protocols for generating sequencing libraries.
  • Building effective new approaches to data analysis.

Main Results:

  • Cost of DNA sequencing reduced by over two orders of magnitude.
  • Sequencing capacity now available to individual investigators.
  • Enabling comprehensive analysis of genomes, transcriptomes, and interactomes.

Conclusions:

  • Next-generation sequencing democratizes biological research.
  • It has the potential to significantly accelerate biological and biomedical discoveries.
  • Widespread adoption of these technologies is expected.