Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hormone Replacement Cycle Frozen-Thawed Embryo Transfer Is Associated With Elevated Perinatal Risk Compared With Natural Ovulatory Cycle Frozen-Thawed and Fresh Embryo Transfers: Retrospective Analysis of 7,593 Live Birth Cycles.

Reproductive medicine and biology·2026
Same author

Multicenter study of fetal crown-rump length measurement using 3D ultrasound in assisted reproductive technology pregnancies.

Journal of medical ultrasonics (2001)·2026
Same author

TUBB8 variants in infertile Japanese women, including a novel homozygous nonsense variant causing zygote multinucleation.

Reproductive biomedicine online·2026
Same author

Niacin improves developmental ability by increasing glutathione levels and promoting male pronuclear formation in porcine oocytes grown in vitro.

The Journal of reproduction and development·2026
Same author

Exploratory evaluation of the bacterial load of uterine microbiota: Potential implications for implantation and treatment response in assisted reproductive technology.

Journal of reproductive immunology·2026
Same author

Centrosomes regulate pronuclear apposition ensuring parental genome unification in cattle.

Reproduction (Cambridge, England)·2025

Related Experiment Video

Updated: Jun 29, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Intensity range extension method for three-dimensional shape measurement in phase-measuring profilometry using a

Shien Ri1, Motoharu Fujigaki, Yoshiharu Morimoto

  • 1Department of Nanomechanics, Graduate School of Engineering, Tohoku University, Aoba 6-6-01, Aramaki, Aoba-ku, Sendai 980-8579, Japan. rishien@ism.mech.tohoku.ac.jp

Applied Optics
|October 11, 2008
PubMed
Summary

This study introduces a new method using a digital micromirror device (DMD) camera to improve 3D shape measurements. It significantly reduces errors from underexposed or overexposed areas in phase-measuring profilometry.

More Related Videos

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Related Experiment Videos

Last Updated: Jun 29, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

  • Optical Metrology
  • 3D Imaging
  • Computer Vision

Background:

  • Phase-measuring profilometry is a key technique for 3D shape and deformation analysis.
  • Conventional methods struggle with objects exhibiting wide reflectance variations, leading to underexposed or overexposed regions.
  • These problematic areas hinder accurate phase analysis and 3D reconstruction.

Purpose of the Study:

  • To develop an intensity range extension method for phase-measuring profilometry.
  • To enhance the accuracy of 3D shape measurements for objects with challenging reflectance properties.
  • To overcome limitations of conventional methods in handling underexposed and overexposed areas.

Main Methods:

  • A novel intensity range extension technique utilizing a digital micromirror device (DMD) camera.
  • Programming inverse intensity patterns based on object reflectance to capture high-contrast, phase-shifted fringe patterns.
  • Direct correspondence between DMD mirrors and CCD pixels for precise optical control.

Main Results:

  • The proposed method significantly extends the measurable intensity range.
  • Achieved higher accuracy in 3D shape measurements compared to conventional techniques.
  • Reduced the percentage of invalid measurement points from 20% to 1% in experimental analyses.
  • Successfully measured complex objects like a metallic art replica and a flat plane.

Conclusions:

  • The DMD-based intensity range extension method effectively addresses underexposure and overexposure issues in phase-measuring profilometry.
  • This technique offers a substantial improvement in the accuracy and reliability of 3D shape measurements.
  • The method demonstrates practical viability and significant advantages for analyzing objects with wide reflectance variations.