Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Shafts: Problem Solving01:09

Transmission Shafts: Problem Solving

Designing a solid shaft that transmits power from a motor to a machine tool involves a series of calculations to ensure the shaft can withstand the stresses applied by bending moments and torques. First, calculate the torque exerted on the gear, considering the power transmitted by the shaft and its rotational speed. Following this, compute the tangential forces acting on the gears, which directly relate to the torque and the gear radius.
Next, use bending moment diagrams for the shaft to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Full-field transmission-type angle-deviation optical microscope with reflectivity-height transformation.

Biomedical optics express·2015
Same author

High Vertical Resolution Full-Field Reflection-Type Three-Dimensional Angle-Deviation Microscope with Nonlinear Error Compensation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2015
Same author

Small displacement measurements based on an angular-deviation amplifier and interferometric phase detection.

Applied optics·2015
Same author

Nonscanning three-dimensional optical microscope based on the reflectivity-height transformation for biological measurements.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2013
Same author

Small-displacement sensing system based on multiple total internal reflections in heterodyne interferometry.

Applied optics·2009
Same author

High-sensitivity small-angle sensor based on surface plasmon resonance technology and heterodyne interferometry.

Applied optics·2006
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
10:25

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope

Published on: September 14, 2018

Transmission-type angle deviation microscopy.

Ming-Hung Chiu1, Chih-Wen Lai, Chen-Tai Tan

  • 1Department of Electro-Optical Engineering, National Formosa University, 64 Wunhua Road, Huwei Yunlin 632, Taiwan. mhchiu@nfu.edu.tw

Applied Optics
|October 11, 2008
PubMed
Summary
This summary is machine-generated.

We developed transmission angle deviation microscopy (TADM), a new 3D optical microscopy method. TADM offers high axial resolution and large measurement ranges for non-contact, nondestructive imaging without sample conductivity or pretreatment.

More Related Videos

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)

Published on: October 2, 2012

Related Experiment Videos

Last Updated: Jun 29, 2026

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
10:25

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope

Published on: September 14, 2018

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)

Published on: October 2, 2012

Area of Science:

  • Optical microscopy
  • Nanotechnology
  • Surface science

Background:

  • Existing microscopy techniques face limitations in axial resolution and measurement range.
  • Nondestructive and noncontact imaging methods are crucial for delicate samples.

Purpose of the Study:

  • To introduce a novel microscopy technique, transmission angle deviation microscopy (TADM).
  • To enhance axial resolution and expand dynamic measurement ranges in 3D optical microscopy.

Main Methods:

  • Utilizes common-path heterodyne interferometry and geometrical optics.
  • Incorporates an ultrahigh sensitivity surface plasmon resonance (SPR) angular sensor.
  • Analyzes beam convergence/divergence due to refractive index or surface height variations.

Main Results:

  • Achieved high axial resolution of approximately 32 nm.
  • Demonstrated large measurement ranges of +/- 80 micrometers with a numerical aperture of 0.21.
  • Enabled non-destructive, non-contact measurement in transparent media without conductivity or pretreatment.

Conclusions:

  • TADM provides significant advantages in axial resolution and measurement range for 3D optical microscopy.
  • The technique is versatile, applicable to samples requiring non-destructive and non-contact analysis.
  • TADM overcomes limitations of traditional methods, offering new possibilities in microscopic imaging.