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Production and partial purification of alpha-amylase from a novel isolate Streptomyces gulbargensis
Dastager G Syed1, Dayanand Agasar, Ashok Pandey
1Biotechnology Division, National Institute of Interdisciplinary Science and Technology (CSIR), Industrial Estate, Pappanamcode, Thiruvananthapuram, 695019, India. syed_micro@rediffmail.com
Abstract:
Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45 degrees C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating alpha-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.
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