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Updated: Jun 29, 2026
12:23
Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
Published on: August 2, 2018
Quantitative phosphoproteomics by mass spectrometry: past, present, and future
Aleksandra Nita-Lazar1, Hideshiro Saito-Benz, Forest M White
1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Proteomics
|October 11, 2008
Summary
Recent technological advances enable deeper analysis of protein phosphorylation signaling networks. Further research is needed to fully understand their role in cellular responses and disease.
Area of Science:
- Cellular Biology
- Molecular Biology
- Biochemistry
Background:
- Protein phosphorylation networks control cellular responses to stimuli.
- Dysregulation of these networks is implicated in various diseases.
- Comprehensive phosphoproteome analysis and network-level signaling monitoring remain challenging.
Purpose of the Study:
- To review technological advancements in phosphoproteomics over the past decade.
- To highlight applications of these tools in uncovering signaling networks.
- To discuss future directions for linking phosphorylation analysis with biological insight.
Main Methods:
- Review of technological advancements in phosphoproteomics.
- Analysis of current applications in diverse biological settings.
- Discussion of future research needs.
Main Results:
- Significant progress in analyzing and quantifying protein phosphorylation events.
- Emerging tools enable better monitoring of signaling networks.
- Current limitations exist in comprehensive phosphoproteome analysis and network-level interpretation.
Conclusions:
- Technological progress has enhanced the study of phosphorylation signaling.
- Further advancements are crucial for a complete understanding of phosphorylation's role in biology and disease.
- Integrating quantitative data with biological context is key for future discoveries.
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