Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning the viscoelasticity of sugar-silicone polymers using caramelization.

Chemical communications (Cambridge, England)·2026
Same author

Prepuberty exposure to polystyrene nanoplastics induces cardiac inflammation through calcium overload-mediated ROS/JAK1/STAT3 signaling cascade.

Free radical biology & medicine·2025
Same author

Strategies to Improve the Sustainability of Silicone Polymers.

Macromolecules·2025
Same author

Risk associated circulating biomarkers S100A3 identified in congenital heart disease-associated pulmonary arterial hypertension.

Intractable & rare diseases research·2025
Same author

Bacteriophage-Activated DNAzyme Hydrogels Combined with Machine Learning Enable Point-of-Use Colorimetric Detection of Escherichia coli.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

A Fe/Zn Dual Single-Atom Nanozyme with High Peroxidase Activities for Detection of Penicillin G.

Analytical chemistry·2024

Related Experiment Video

Updated: Jun 29, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Paper-based bioassays using gold nanoparticle colorimetric probes.

Weian Zhao1, M Monsur Ali, Sergio D Aguirre

  • 1Department of Chemistry, School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4M1, Canada.

Analytical Chemistry
|October 14, 2008
PubMed
Summary

Paper-based bioassays using gold nanoparticle (AuNP) colorimetric probes offer a stable, inexpensive, and portable solution for diagnostics. These assays function without refrigeration, making them ideal for resource-limited settings.

More Related Videos

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Related Experiment Videos

Last Updated: Jun 29, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Area of Science:

  • Nanomaterials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Traditional bioassays often rely on thermosensitive reagents and controlled laboratory conditions.
  • Developing regions require affordable, user-friendly diagnostic tests operable without refrigeration or specialized personnel.

Purpose of the Study:

  • To develop a paper-based bioassay platform utilizing gold nanoparticle (AuNP) colorimetric probes for simple, stable, and portable diagnostics.
  • To assess the performance and stability of these paper-based assays under various conditions.

Main Methods:

  • Development of paper substrates spotted with DNA-cross-linked gold nanoparticle (AuNP) aggregates for DNase I and adenosine detection.
  • Evaluation of different paper types (hydrophobic, coated hydrophilic, uncoated hydrophilic) for assay performance.
  • Testing the thermal stability and storage capabilities of the AuNP-spotted paper substrates.

Main Results:

  • Paper-based AuNP colorimetric probes demonstrated rapid (within one minute) color changes for target detection (DNase I or adenosine).
  • Hydrophobic and PVA-coated hydrophilic papers were suitable; uncoated hydrophilic paper led to assay failure.
  • The assays exhibited remarkable thermal stability, withstanding drying at 90°C and maintaining function after weeks of storage.

Conclusions:

  • Paper-based AuNP colorimetric assays are inexpensive, portable, disposable, and easy to use, suitable for resource-limited environments.
  • The paper substrate protects AuNP probes from heat and other stimuli, enhancing assay stability.
  • This platform holds promise for applications in disease diagnostics, pathogen detection, and food/water quality monitoring.