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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Lysogenic Cycle of Bacteriophages00:43

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Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
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Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

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Updated: Jun 29, 2026

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
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T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo

Published on: January 26, 2024

Bacteriophage T5 DNA ejection under pressure.

A Leforestier1, S Brasilès, M de Frutos

  • 1Laboratoire de Physique des Solides, Université Paris-Sud, CNRS UMR 8502, Bât. 510, F-91405 Orsay Cedex, France.

Journal of Molecular Biology
|October 14, 2008
PubMed
Summary
This summary is machine-generated.

Bacteriophage T5 DNA ejection shows unexpected behavior under low pressure, with multiple ejection states observed. This differs from other phages and suggests kinetics or machinery structure influences the process.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biophysics

Background:

  • Bacteriophage genome transfer into host cells is crucial for infection.
  • Bacteriophage T5 DNA ejection is initiated by binding to the Escherichia coli receptor FhuA.

Purpose of the Study:

  • To investigate the effect of external osmotic pressure on bacteriophage T5 DNA ejection.
  • To understand the mechanisms governing T5 DNA ejection and compare it to other bacteriophages.

Main Methods:

  • Electrophoresis
  • Cryo-electron microscopy
  • Controlled osmotic pressure experiments

Main Results:

  • In high pressure (7-16 atm), DNA ejection decreased with increasing pressure, consistent with lambda and SPP1 phages.
  • In low/moderate pressure (2-7 atm), T5 displayed multiple, non-random DNA ejection states, unlike other phages.
  • T5 ejection is not solely explained by pressure equilibrium, suggesting other factors are involved.

Conclusions:

  • Bacteriophage T5 DNA ejection is complex and not solely governed by osmotic pressure.
  • Kinetics and structural features of the T5 ejection machinery likely play a significant role.
  • Further research is needed to elucidate the precise mechanisms of T5 DNA ejection.