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Related Experiment Video

Updated: Jun 29, 2026

Functional Characterization of Endogenously Expressed Human RYR1 Variants
07:59

Functional Characterization of Endogenously Expressed Human RYR1 Variants

Published on: June 9, 2021

Studies of RyR function in situ.

Natalia Shirokova1, Ernst Niggli

  • 1Department of Pharmacology & Physiology, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA. nshiroko@umdnj.edu

Methods (San Diego, Calif.)
|October 14, 2008
PubMed
Summary
This summary is machine-generated.

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Ca2+ signalling in cardiac muscle: the importance of balances.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
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Dual ablation of the RyR2-Ser2808 and RyR2-Ser2814 sites increases propensity for pro-arrhythmic spontaneous Ca<sup>2+</sup> releases.

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The yin and yang of intracellular delivery of amphipathic optical probes using <i>n</i>-butyl charge masking.

Chemical communications (Cambridge, England)·2022

Optical methods allow studying ryanodine receptors (RyRs) in their native environment. This approach investigates intracellular calcium (Ca2+) signals, enhancing our understanding of RyR function in health and disease.

Area of Science:

  • Cellular Biology
  • Physiology
  • Biophysics

Background:

  • Ryanodine receptors (RyRs) are critical intracellular Ca2+ release channels in the sarcoplasmic reticulum.
  • RyRs play a vital role in cellular processes like muscle excitation-contraction coupling.
  • Existing methods for studying RyRs often involve purified channels, losing their natural cellular context.

Purpose of the Study:

  • To review optical methods for studying RyR functions in situ (within their natural cellular environment).
  • To highlight techniques for investigating local intracellular Ca2+ signals, such as Ca2+ sparks.

Main Methods:

  • Confocal microscopy for high-resolution imaging of Ca2+ dynamics.
  • Flash photolysis of caged compounds to trigger rapid Ca2+ release.

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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

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Last Updated: Jun 29, 2026

Functional Characterization of Endogenously Expressed Human RYR1 Variants
07:59

Functional Characterization of Endogenously Expressed Human RYR1 Variants

Published on: June 9, 2021

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

  • In situ analysis of ryanodine receptor activity within the sarcoplasmic reticulum.

    • Main Results:

    • Optical methods provide insights into RyR function in their native cellular milieu.
    • Techniques discussed enable detailed investigation of localized Ca2+ signaling events (Ca2+ sparks).
    • These methods overcome limitations of studying purified RyR channels.

    Conclusions:

    • In situ optical methods are powerful tools for understanding RyR function.
    • Continued application of these techniques will advance knowledge of RyR roles in physiological and pathological states.
    • This approach is crucial for deciphering complex cellular signaling pathways involving Ca2+.