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Use of flow cytometry to monitor Legionella viability

Séverine Allegra1, Françoise Berger, Philippe Berthelot

  • 1Groupe Immunité des Muqueuses et Agents Pathogènes, EA3064, Faculté de Médecine J. Lisfranc, Université Jean Monnet, Saint-Etienne, France.

Applied and Environmental Microbiology
|October 14, 2008
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...