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Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

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Published on: November 17, 2014

Increased Rac2 mRNA expression in peripheral blood during human corneal graft rejection.

M-C Huang¹, A B Tullo, M C Hillarby

¹Department of Medical Genetics, University of Manchester, UK.

Eye (London, England)

|October 14, 2008

Summary

Gene expression in peripheral blood can indicate corneal transplant rejection. Upregulated Rac2 mRNA and decreased RhoA, paxillin, and CD18 mRNA levels were observed in patients experiencing graft rejection.

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Area of Science:

Immunology
Ophthalmology
Molecular Biology

Background:

Allograft rejection is a primary cause of corneal graft failure.
The precise pathomechanisms underlying corneal transplantation rejection remain unclear.

Purpose of the Study:

To compare gene expression profiles in peripheral blood between patients with and without corneal graft rejection.
To identify potential molecular markers associated with corneal transplant rejection.

Main Methods:

Utilized cDNA array technology to survey and quantify transcript expression in 66 corneal transplant patients (18 with rejection).
Employed semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to validate gene expression patterns of selected genes.

Main Results:

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Eight genes exhibited differential expression among 265 analyzed genes.
Rac 2 mRNA was significantly upregulated in the rejection group.
RhoA, paxillin, and CD18 mRNA levels were decreased in patients experiencing graft rejection.

Conclusions:

Peripheral blood gene expression analysis, particularly Rac2 mRNA levels, may serve as a biomarker for corneal transplant rejection.
cDNA array technology is a valuable tool for discovering novel genes involved in corneal graft rejection pathways.