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Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
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Optimal optical trap for bacterial viability.

Utkur Mirsaidov1, Winston Timp, Kaethe Timp

  • 1Beckman Institute, University of Illinois, Urbana, Illinois 61801, USA.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|October 15, 2008
PubMed
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Time-sharing optical traps significantly improves bacterial viability by reducing laser exposure. A lethal energy dose of approximately 5 Joules limits E. coli survival, enabling better control in cell manipulation.

Area of Science:

  • Biophysics
  • Microbiology
  • Optical Engineering

Background:

  • Optical trapping is vital for bacterial micromanipulation.
  • Laser-induced photodamage compromises bacterial viability during optical trapping.

Purpose of the Study:

  • To investigate optical trapping parameters that preserve E. coli viability.
  • To determine optimal conditions for minimizing laser-induced photodamage in bacteria.

Main Methods:

  • Utilizing near-infrared optical traps (840-930 nm) for E. coli manipulation.
  • Assessing bacterial viability via green fluorescent protein gene expression.
  • Comparing continuous wave (CW) versus time-shared optical trap exposure.

Main Results:

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Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

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  • Time-sharing optical traps (10 µs-1 ms dwell time) enhanced E. coli viability compared to CW exposure.
  • Photodamage showed weak dependence on wavelength but linear dependence on peak power.
  • A consistent lethal energy dose of approximately 5 J was identified for E. coli.

    • Conclusions:

    • Time-sharing is a viable strategy to mitigate photodamage in optical trapping of bacteria.
    • Bacterial viability in optical traps is primarily limited by total energy dose, not just peak power or wavelength.
    • Energy dose serves as a critical parameter for controlling bacterial survival during optical manipulation.