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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
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Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
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Drugs for Treatment of Ulcerative Colitis in IBD

Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide generation. 

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Related Experiment Video

Updated: Jun 29, 2026

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
11:13

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Published on: June 9, 2023

Acetylsalicylic acid and salicylic acid decrease tumor cell viability and glucose metabolism modulating

Guilherme A Spitz1, Cristiane M Furtado, Mauro Sola-Penna

  • 1Laboratório de Oncobiologia Molecular (LabOMol), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil.

Biochemical Pharmacology
|October 15, 2008
PubMed
Summary

Acetylsalicylic acid (ASA) and salicylic acid (SA) inhibit 6-phosphofructo-1-kinase (PFK), a key enzyme in cancer cell glycolysis. This drug-induced PFK inhibition reduces cancer cell glucose consumption and viability, suggesting a novel anti-tumoral mechanism.

Related Experiment Videos

Last Updated: Jun 29, 2026

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
11:13

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Published on: June 9, 2023

Area of Science:

  • Biochemistry
  • Cancer Biology
  • Pharmacology

Background:

  • Cancer cells exhibit elevated glycolysis compared to normal cells, making glycolysis a target for anti-cancer drug development.
  • Anti-inflammatory drugs like acetylsalicylic acid (ASA) and salicylic acid (SA) show anti-tumoral effects, including apoptosis induction and altered glucose metabolism.

Purpose of the Study:

  • To investigate if ASA can directly reduce cancer cell glycolysis by inhibiting 6-phosphofructo-1-kinase (PFK).
  • To elucidate the mechanism of PFK inhibition by ASA and SA.

Main Methods:

  • In vitro inhibition assays of purified PFK by ASA and SA.
  • Analysis of enzyme quaternary structure changes upon drug binding.
  • Assessment of glucose consumption and cell viability in MCF-7 breast cancer cells.

Main Results:

  • ASA and SA demonstrated dose-dependent inhibition of purified PFK.
  • Inhibition was attributed to the dissociation of active PFK tetramers into inactive dimers, a known regulatory mechanism.
  • Drug effects were reversible and could be modulated by actin filament binding.
  • ASA and SA decreased glucose uptake and viability in MCF-7 cells, correlating with PFK inhibition.

Conclusions:

  • ASA and SA directly inhibit PFK, a key regulatory enzyme in glycolysis.
  • This direct enzyme modulation is proposed as a mechanism underlying the anti-tumoral effects of ASA and SA.
  • The findings highlight PFK as a potential target for anti-cancer therapies using these anti-inflammatory drugs.