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Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

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Published on: February 24, 2023

Fibre based cellular transfection.

X Tsampoula1, K Taguchi, T Cizmár

  • 1SUPA, School of Physics & Astronomy, University of St Andrews, North Haugh, St Andrews, Scotland, UK. xt3@st-andrews.ac.uk

Optics Express
|October 15, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fiber-optic system for optically assisted cell transfection, achieving high efficiencies comparable to free-space methods. This innovation enables new applications in tissue transfection and endoscopic procedures.

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Published on: December 28, 2015

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Optics and Photonics

Background:

  • Optically assisted transfection uses lasers to deliver therapeutic agents into cells.
  • Current methods rely on free-space laser beams, limiting applications.
  • Ultrashort pulse lasers enable transient cell membrane permeation via multiphoton processes.

Purpose of the Study:

  • To introduce the first fiber-delivered system for optical cell transfection.
  • To engineer an optical fiber with an axicon tip for enhanced remote field intensity.
  • To develop a theoretical model for predicting light propagation and optimizing transfection.

Main Methods:

  • Standard optical fiber engineered with an axicon tip.
  • Delivery of ultrashort (~800 fs) laser pulses.
  • Theoretical modeling of light propagation in air and water.
  • Experimental validation of the fiber-optic system.

Main Results:

  • Fiber-optic system achieves efficient optical transfection without close proximity to cells.
  • Theoretical model accurately predicts experimental findings for axicon and bare fibers.
  • Transfection efficiencies up to 57% achieved, comparable to free-space methods.

Conclusions:

  • Fiber-optic delivery represents a significant advancement in optical transfection technology.
  • The developed system and model facilitate optimization for cellular transfection.
  • This breakthrough enables potential applications in tissue transfection and endoscopic techniques.