Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π&VeryThinSpace;→&VeryThinSpace;π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Preclinical Safety and Feasibility Study of Line-Field Confocal Optical Coherence Tomography for Ophthalmology Applications.

Translational vision science & technology·2026

Same author

Metabolic imaging of <i>Fragilariopsis cylindrus</i> in polar night conditions using full-field optical transmission tomography (FFOTT).

Biomedical optics express·2026

Same author

Substantial aircraft contrail formation at low soot emission levels.

Nature·2026

Same author

Plasmonic Film with Diluted Nanostructures for Light Energy Harvesting and Sensing.

ACS applied optical materials·2025

Same author

Impact of Scattering Variation Measured by Line-Field Confocal Optical Coherence Tomography on Fluorescence Measurement by Optical Spectroscopy: A Study on Phantoms and Human Skin Models.

Skin pharmacology and physiology·2025

Same author

AI-assisted identification of nonmelanoma skin cancer structures based on combined line-field confocal optical coherence tomography and confocal Raman microspectroscopy.

Journal of biomedical optics·2025

Search research articles

Related Experiment Video

Updated: Jun 29, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Spectroscopic ultrahigh-resolution full-field optical coherence microscopy.

Arnaud Dubois¹, Julien Moreau, Claude Boccara

¹Laboratoire Charles Fabry de l'Institut d'Optique, CNRS UMR 8501, Université Paris-Sud, Campus Polytechnique, Palaiseau, France. arnaud.dubois@institutoptique.fr

|October 15, 2008

Summary

We developed a novel optical coherence microscopy system for high-resolution imaging. This technique provides detailed tomographic and spectroscopic data, enhancing contrast for biological tissue visualization.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Related Experiment Videos

Last Updated: Jun 29, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

Biomedical Optics
Microscopy
Spectroscopy

Background:

Optical coherence microscopy (OCM) offers label-free imaging capabilities.
High spatial resolution is crucial for detailed microstructural analysis.
Simultaneous tomographic and spectroscopic information can improve diagnostic accuracy.

Purpose of the Study:

To develop a full-field optical coherence microscopy system.
To achieve ultrahigh spatial resolution for tomographic and spectroscopic imaging.
To demonstrate the system's capability for biological tissue imaging.

Main Methods:

Utilized a Linnik-type microscope for image acquisition.
Employed short-time Fourier analysis on en face interferometric images.
Used a halogen lamp for spectroscopic imaging across 600-900 nm.

Main Results:

Achieved spatial resolution of approximately 1 micrometer.
Successfully performed absorption measurements on a colored gel.
Demonstrated enhanced contrast in imaging a Xenopus Laevis tadpole.

Conclusions:

The developed OCM system provides high-resolution tomographic and spectroscopic imaging.
The technique is validated for absorption measurements and biological tissue contrast enhancement.
This system holds potential for advanced biomedical imaging applications.