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Related Experiment Video

Updated: Jun 29, 2026

Isolation and Characterization of RNA-Containing Exosomes

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

Dendritic cells release HLA-B-associated transcript-3 positive exosomes to regulate natural killer function.

Venkateswara Rao Simhadri¹, Katrin S Reiners, Hinrich P Hansen

¹Laboratory of Immune Therapy, Department of Internal Medicine I, Centre for Integrated Oncology Koeln Bonn, University of Cologne, Cologne, Germany. venkatsimhadri@googlemail.com

|October 15, 2008

Summary

Natural Killer cell receptor NKp30 binds to the intracellular protein BAT3 presented on exosomes. This interaction mediates Natural Killer and dendritic cell crosstalk, influencing cell killing and maturation, offering new therapeutic strategies.

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Isolation and Characterization of RNA-Containing Exosomes

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Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

NKp30 is a natural cytotoxicity receptor on NK cells crucial for anti-tumor activity and regulating dendritic cells (DCs).
BAT3, an intracellular protein involved in apoptosis, was recently identified as an NKp30 ligand.
Mechanisms of BAT3 surface exposure and its role in NK-DC crosstalk were unclear.

Purpose of the Study:

To elucidate the mechanism of exosomal BAT3 presentation and its functional role in NK-DC interactions.
To investigate the impact of BAT3 on NK cell activation and cytokine release.
To determine the BAT3-dependency of NKp30-mediated NK/DC crosstalk.

Main Methods:

Electron microscopy and flow cytometry to detect surface BAT3 on exosomes.
Overexpression and depletion of BAT3 in 293T cells.
Assessment of NK cell cytokine release upon interaction with BAT3-expressing exosomes.
Evaluation of NK-mediated iDC killing and maturation.

Main Results:

Exosomes from 293T cells and immature dendritic cells (iDCs) display surface-expressed BAT3, recognized by NKp30-Ig.
BAT3 levels on exosomes directly correlate with NK cell activation and cytokine release.
NKp30-mediated killing and maturation of iDCs are dependent on BAT3.

Conclusions:

A novel mechanism of NK cell activation via exosomal BAT3 released from accessory cells is proposed.
BAT3-carrying exosomes mediate NK-DC crosstalk, influencing DC maturation and cytotoxicity.
Targeting exosomal BAT3 may provide novel strategies for cancer immunotherapy and managing autoimmune diseases.