Related Experiment Video
Updated: Jun 29, 2026
Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium
Published on: April 6, 2022
In vivo confocal microscopic grading system for standardized corneal evaluation: application to toxic-induced damage
Aude Pauly1, Antoine Labbe, Christophe Baudouin
1Paris-Descartes University, Paris, France. aude pauly@yahoo.com
Purpose:
To propose an in vivo confocal microscopic scoring system for evaluation of irritant-induced corneal changes.
Materials And Methods:
Rat corneas were instilled with 0.01-0.5% benzalkonium chloride (BAC) and examined using high-resolution in vivo confocal microscopy (HRT-II) to measure corneal thickness and characterize corneal damage patterns. Severity scores were given for each predefined evaluation parameter and then totaled.
Results:
The scoring system revealed a dose-dependent effect of BAC and discriminated between high- and low-dose treatments.
Conclusions:
This HRT-II scoring standardizes damage evaluation at the cellular level, even when assessing irritating compounds at low concentrations.
