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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

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Published on: January 26, 2013

In vitro characterization of embryionic ST14A-cells.

Marine Hovakimyan¹, Karola Weinreich, Stefan Jean-Pierre Haas

¹Institute of Anatomy, University of Rostock, Rostock, Germany.

The International Journal of Neuroscience

|October 15, 2008

Summary

The ST14A cell line shows temperature-dependent neural progenitor cell differentiation. Culturing at 39°C promotes neuronal marker expression, suggesting potential for restorative therapies.

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Area of Science:

Neuroscience
Developmental Biology
Cell Biology

Background:

The ST14A cell line is an immortalized embryonic striatal cell line.
Understanding neural progenitor cell differentiation is crucial for regenerative medicine.

Purpose of the Study:

To characterize the temperature-sensitive differentiation potential of the ST14A cell line.
To evaluate ST14A cells as a model for studying neuronal development and for potential therapeutic applications.

Main Methods:

Immunocytochemistry was used to analyze marker expression in ST14A cells.
Cells were cultured in vitro at permissive (33°C) and non-permissive (39°C) temperatures for up to 14 days.
Differentiation was assessed in standard and serum-free media.

Main Results:

At 33°C, ST14A cells proliferated and expressed neural progenitor markers (nestin, vimentin) but not neuronal markers.
At 39°C, proliferation was delayed, and cells increased neuronal marker expression while decreasing progenitor markers.
Serum-free conditions enhanced the process of neuronal differentiation.

Conclusions:

The ST14A cell line exhibits temperature-inducible neuronal differentiation.
These findings highlight the potential of ST14A cells for experimental restorative therapies in neuroscience.
Temperature control offers a method to modulate neural progenitor cell fate in vitro.