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Fabrication of a layered microstructured polycaprolactone construct for 3-D tissue engineering.

Sumona Sarkar1, Brett C Isenberg, Eran Hodis

  • 1Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA.

Journal of Biomaterials Science. Polymer Edition
|October 16, 2008
PubMed
Summary

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Researchers created 3-D tissue scaffolds using microgrooved polycaprolactone (PCL) films. These scaffolds promote cell organization and stability, enabling studies on tissue-engineered blood vessels.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue scaffolds require cellular organization and functional properties for regeneration.
  • Previous studies demonstrated 2-D microgrooved substrates guide cell and ECM alignment.
  • Transferring microtopography to 3-D constructs is crucial for advanced tissue mimics.

Purpose of the Study:

  • To fabricate 3-D tissue scaffolds using microgrooved biodegradable polycaprolactone (PCL) films.
  • To develop a layering technique for cellularized microtextured scaffolds into a 3-D construct.
  • To investigate the impact of precise cellular organization on tissue-engineered blood vessel properties.

Main Methods:

  • Microgrooves were fabricated on PCL thin films.

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  • A surface modification technique attached photoreactive groups to PCL.
  • Poly(ethylene glycol)-diacrylate (PEG-DA) gel was photopolymerized as an adhesive layer between PCL scaffolds.
  • XPS confirmed PCL surface modification; colorimetric and imaging techniques assessed cell number and orientation.

    • Main Results:

    • Microtopography was successfully transferred to biodegradable PCL films.
    • A novel layering technique created stable, organized 3-D composite structures.
    • Cell number and orientation were maintained on modified PCL and within the 3-D construct.
    • The PEG-DA layer effectively adhered cellularized PCL scaffolds.

    Conclusions:

    • A method for creating 3-D tissue mimics with controlled cellular organization was established.
    • The developed scaffolds provide a platform for studying the effects of cell orientation on tissue function.
    • This approach advances the development of tissue-engineered blood vessels with improved mechanical and biological properties.