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Related Experiment Videos

Mutagenic DNA repair in enterobacteria.

S G Sedgwick1, C Ho, R Woodgate

  • 1Genetics Division, National Institute for Medical Research, London, Great Britain.

Journal of Bacteriology
|September 1, 1991
PubMed
Summary

Mutagenic DNA repair, a process in bacteria like Escherichia coli, is more widespread in enterobacteria than previously believed. This DNA repair system, encoded by umuDC genes, shows variations in activity and complexity across species.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Mutagenic DNA repair in Escherichia coli is mediated by the umuDC operon.
  • UmuD and UmuC protein synthesis is induced during the SOS response to DNA damage.
  • UmuD protein undergoes cleavage to UmuD' for induced mutagenesis.

Purpose of the Study:

  • To investigate the distribution and characteristics of mutagenic DNA repair systems in sixteen enterobacteria species.
  • To determine if similar umuDC-like genes and proteins are present and functional in other enteric bacteria.
  • To explore the variability in UV-induced mutability and umu gene complexity across different species.

Main Methods:

  • Inducible-mutagenesis assays were performed on sixteen enterobacteria species.
  • Cross-reactivity with E. coli UmuD and UmuD' antibodies was used to detect UmuD-like proteins.
  • Hybridization with E. coli and Salmonella typhimurium umu DNA probes was employed to map umu-like genes.

Main Results:

  • Mutagenic DNA repair systems are more widely distributed among enterobacteria than previously thought.
  • umu loci in other species can be more complex than the umuDC operon in E. coli.
  • Significant variations in UV-induced mutability (over 200-fold) were observed between species and even E. coli isolates.

Conclusions:

  • The umuDC system's presence suggests a conserved mechanism for DNA repair across enterobacteria.
  • Variations in mutability despite the presence of umu-like genes indicate potential regulation or alternative functions.
  • umuDC genes may be retained for functions beyond mutagenesis, possibly in an unknown process.

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