Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same author

Assessment of Papillary Thyroid Carcinoma by Profiling Multiple Matrix Metalloproteinase Activities Using a Machine Learning-Assisted Peptide Microarray Sensing Platform.

Analytical chemistry·2026
Same author

Clinical efficacy of cryopreserved autologous bone flaps versus titanium plates for cranioplasty: a retrospective comparative study.

The International journal of neuroscience·2026
Same author

Biosynthetic Gene Cluster Diversity and Species-Specific Metabolic Potential in Ustilaginaceae.

Journal of fungi (Basel, Switzerland)·2026
Same author

Multiomics Identification of Radioresistance-Associated Biomarkers and Prognostic Model Construction in Rectal Cancer.

Human mutation·2026
Same author

Copper-hollow mesoporous polydopamine-polyacrylic acid nanoplatform enabling photoacoustic imaging-guided photothermal/chemodynamic therapy for osteosarcoma.

RSC advances·2026

Related Experiment Video

Updated: Jun 29, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Microarray-based study of carbohydrate-protein binding by gold nanoparticle probes.

Jingqing Gao1, Dianjun Liu, Zhenxin Wang

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

Analytical Chemistry
|October 16, 2008
PubMed
Summary

We developed a new high-throughput tool for studying carbohydrate-protein interactions using microarrays and resonance light scattering. This method enables sensitive detection of these crucial biological interactions.

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Related Experiment Videos

Last Updated: Jun 29, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Carbohydrate-protein interactions are vital in biological processes.
  • High-throughput tools are needed for efficient monitoring of these interactions.

Purpose of the Study:

  • To develop a novel high-throughput tool for monitoring carbohydrate-protein interactions.
  • To establish a resonance light scattering (RLS) assay for microarray-based detection.

Main Methods:

  • Preparation of carbohydrate or glycoprotein microarrays.
  • Immobilization of biomolecules on functionalized glass slides.
  • Detection of lectin binding using gold nanoparticles, silver deposition, and RLS.

Main Results:

  • Demonstrated highly selective recognition of carbohydrate-protein interactions.
  • Achieved sensitive detection limits: 25.6 pg/mL for RCA120, 8 µM for Gal-β, and 32 ng/mL for Asf.
  • Successfully established the RLS assay using well-defined recognition systems.

Conclusions:

  • The developed microarray-based RLS assay is a powerful tool for high-throughput monitoring of carbohydrate-protein interactions.
  • The method offers high sensitivity and selectivity for detecting these interactions.
  • This tool has potential applications in diagnostics and drug discovery.