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Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

Bone Marrow Sampling and Transplants

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Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

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Updated: Jun 29, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

SAGE application in hematological research.

Shin-ichi Hashimoto¹, Kouji Matsushima

¹Department of Molecular Preventive Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. hashimot@m.u-tokyo.ac.jp

Current Pharmaceutical Biotechnology

|October 16, 2008

Summary

This summary is machine-generated.

Serial analysis of gene expression (SAGE) reveals molecular details of blood cell subsets. This review analyzes SAGE data to define the function and subsets of various blood cells.

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Last Updated: Jun 29, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Area of Science:

Hematology
Genomics
Immunology

Background:

Blood cells are crucial for homeostasis and defense against pathogens, cancer, and autoimmune diseases.
Leukocyte subsets interact via surface molecules, and advancements in genomics have accelerated the identification of these molecules.
Functional genomics technologies like DNA microarray and SAGE enable large-scale gene expression analysis.

Purpose of the Study:

To molecularly define blood cell subsets and their functions.
To review Serial Analysis of Gene Expression (SAGE) data from various hematopoietic cells.
To elucidate cell characteristics based on stage-specific gene expression.

Main Methods:

Utilized Serial Analysis of Gene Expression (SAGE) for quantitative gene expression analysis.
Performed SAGE on diverse hematopoietic cell types at various differentiation and activation stages.
Compiled and reviewed existing SAGE data from multiple research groups.

Main Results:

SAGE provides a quantitative and comprehensive analysis of gene expression in blood cells.
Gene expression profiles differ across various blood cell types and developmental stages.
Identified key genes involved in cell-cell interactions, activation, and differentiation.

Conclusions:

SAGE is a powerful tool for molecularly defining blood cell subsets and functions.
Comprehensive gene expression analysis aids in understanding blood cell characteristics.
The reviewed SAGE data provides valuable insights into hematopoiesis and immune responses.