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Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase
M M Van Lookeren Campagne1, J Franke, R H Kessin
1Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
The Journal of Biological Chemistry
|September 5, 1991
Abstract:
A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with a predicted molecular mass of 79.6 kDa. The Dictyostelium enzyme has extensive homology with the GMP synthetase of Escherichia coli and regional homology to other glutamine amidotransferases.