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Transcription factor PDX-1 in human colorectal adenocarcinoma: a potential tumor marker?
Nikiforos Ballian1, Shi-He Liu, Francis-Charles Brunicardi
1The Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
Aim:
To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer.
Methods:
RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient.
Results:
The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue.
Conclusion:
These data argue for further evaluation of PDX-1 as a biomarker for colorectal cancer.
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