Related Experiment Videos
Isotachophoretic determination of 2-5A phosphodiesterase
G Bruchelt1, M Buedenbender, K H Schmidt
1Children's Hospital, University of Tübingen, Germany.
Journal of Chromatography
|June 7, 1991
Abstract:
Together with 2-5A synthetase and ribonuclease L, 2-5A phosphodiesterase belongs to the 2-5A system, which plays an important role in the action of interferon. Analytical capillary isotachophoresis was used for the determination of 2-5A phosphodiesterase activity. Enzyme assay was optimized using snake venom phosphodiesterase as a source of 2-5A phosphodiesterase activity. The 2-5A trimer core was used as a substrate. Enzyme activity was determined in time- and concentration-dependent reactions. In addition, 2-5A phosphodiesterase activity was determined in lysates of mononuclear blood cells.