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Antirival activity of N-phenyl-N'-arylthiourea derivatives against some Rhinoviruses
Abstract:
The effect of 12 derivatives of N-phenyl-N'-aryl- or alkylthiourea, inhibitors of human enteroviruses and foot-and-mouth disease virus, on reproduction of some rhinoviruses (H-17, B-632) in HeLa Bristol cells was studied. As screening methods both the multicylce growth test in roller tube cultures and two variants of plaque inhibition tests were employed. The compounds selected were tested in one-step growth cycle set-up. We established that N-phenyl-N'-4-hydroxyphenylthiourea (V-24) and N-phenyl-N'-2-carboxyphenylthiourea (V-17) have a distinct inhibitory effect on the growth of rhinovirus H-17, and N-phenyl-N'-2-hydroxy-5-nitrophenylthiourea (V-25) inhibited strongly the multiplication of rhinovirus B-632.
Insights
Twelve thiourea derivatives were evaluated for their antiviral activity against rhinoviruses. Specific compounds demonstrated significant inhibition of rhinovirus H-17 and rhinovirus B-632 replication in cell cultures.
Area of Science:
- Virology
- Medicinal Chemistry
- Cell Biology
Background:
- Rhinoviruses are a major cause of the common cold and can lead to more severe respiratory illnesses.
- Existing antiviral therapies for rhinoviruses are limited, necessitating the search for novel therapeutic agents.
- Thiourea derivatives have shown promise as inhibitors of various viruses, including enteroviruses and foot-and-mouth disease virus.
Purpose of the Study:
- To investigate the antiviral potential of N-phenyl-N'-aryl- or alkylthiourea derivatives against specific rhinovirus strains.
- To identify novel compounds effective in inhibiting rhinovirus replication in a cellular model.
Main Methods:
- Screening of 12 thiourea derivatives using multicyle growth tests and plaque inhibition assays in HeLa Bristol cells.
- Evaluation of selected compounds in a one-step growth cycle assay to confirm inhibitory effects.
- Testing against rhinovirus H-17 and rhinovirus B-632.
Main Results:
- N-phenyl-N'-4-hydroxyphenylthiourea (V-24) and N-phenyl-N'-2-carboxyphenylthiourea (V-17) exhibited significant inhibition of rhinovirus H-17.
- N-phenyl-N'-2-hydroxy-5-nitrophenylthiourea (V-25) demonstrated strong inhibition of rhinovirus B-632 multiplication.
- The study identified specific thiourea derivatives with potent antiviral activity against tested rhinovirus serotypes.
Conclusions:
- Certain N-phenyl-N'-aryl- or alkylthiourea derivatives possess significant antiviral activity against rhinoviruses.
- Compounds V-24, V-17, and V-25 are promising candidates for further development as rhinovirus inhibitors.
- These findings contribute to the development of new strategies for managing rhinovirus infections.