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5-HT receptors: subtypes and second messengers.
1Preclinical Research, SANDOZ Pharma Ltd, Basel, Switzerland.
Journal of Receptor Research
|January 1, 1991
Summary
Recent advances in molecular biology and ligand discovery have significantly expanded our understanding of serotonin (5-hydroxytryptamine, 5-HT) receptors. This progress has led to the identification of numerous receptor subtypes and families.
Area of Science:
- Neuroscience and Pharmacology
- Molecular Biology
- Receptor Research
Background:
- Significant advancements in understanding serotonin (5-hydroxytryptamine, 5-HT) receptors have occurred recently.
- The development of selective ligands and novel techniques has greatly increased the number of recognized receptor subtypes.
Purpose of the Study:
- To review recent developments in the characterization of serotonin (5-HT) receptor subtypes.
- To highlight the impact of new techniques on receptor research.
Main Methods:
- Radioligand binding studies
- Autoradiography
- Second messenger analysis
- Molecular biological techniques, including cDNA cloning
Main Results:
- Three main families of 5-HT receptors with described subtypes are now accepted.
- Molecular biological techniques, particularly cDNA cloning, have substantiated the heterogeneity of 5-HT receptors.
Conclusions:
- Current knowledge of 5-HT receptor subtypes has been significantly enhanced by recent research.
- The integration of various techniques has been crucial for characterizing receptor heterogeneity.