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Related Experiment Videos

Failure to detect platelet-activating factor using the splenectomized mouse bioassay.

J M Scodras1, K J Betteridge, B A Croy

  • 1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.

Journal of Reproduction and Fertility
|July 1, 1991
PubMed
Summary
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Platelet-activating factor (PAF) did not cause thrombocytopaenia or activate platelets in common mouse strains. These findings indicate many mouse strains are unsuitable for PAF bioassays.

Area of Science:

  • Hematology
  • Immunology
  • Pharmacology

Background:

  • Platelet-activating factor (PAF) is a potent lipid mediator involved in inflammation and thrombosis.
  • The bioactivity of PAF is typically assessed by its ability to induce thrombocytopaenia (a decrease in platelet count) and platelet aggregation.
  • Mouse models are frequently used for studying PAF's effects, but their suitability requires validation.

Purpose of the Study:

  • To evaluate the responsiveness of mouse platelets to platelet-activating factor (PAF) in vitro and in vivo.
  • To determine if common mouse strains are suitable for PAF bioassays.
  • To investigate potential reasons for unresponsiveness to PAF in mice.

Main Methods:

  • Purified platelet-activating factor (PAF) from three suppliers was used.

Related Experiment Videos

  • PAF's ability to induce thrombocytopaenia was tested in splenectomized CD1 and Swiss Webster mice.
  • Platelet aggregation assays were performed in vitro using platelets from various mouse strains, stimulated with PAF, adenosine diphosphate, and thrombin.
  • Circulating platelet concentrations were monitored during early gestation in intact and splenectomized mice.

    • Main Results:

    • PAF preparations potently activated platelets from horses and cows in vitro.
    • Injections of PAF did not induce thrombocytopaenia in CD1 or Swiss Webster mice.
    • Mouse platelets from tested strains were unresponsive to PAF in vitro but responded to adenosine diphosphate and thrombin.
    • Thrombin injection caused a dose-dependent decrease in platelet concentration in Swiss Webster mice.
    • No changes in platelet counts were observed during early pregnancy in intact or splenectomized mice.

    Conclusions:

    • Many common mouse strains exhibit refractoriness to platelet-activating factor (PAF).
    • Mouse platelets are generally insensitive to PAF in vitro, despite responsiveness to other agonists.
    • These findings suggest that many mouse strains are unsuitable for use in platelet-activating factor (PAF) bioassays.
    • Further research may be needed to identify responsive mouse models or alternative bioassay methods for PAF.