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Maintenance of CNS tissues in vitro for subsequent pharmacologic evaluation: a simple and inexpensive superfusion
1Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77550.
Journal of Pharmacological Methods
|July 1, 1991
Abstract:
A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologic action of serotonin in the midbrain dorsal raphe nucleus are discussed.