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Updated: Sep 2, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Clean-up and determination of sodium valproate in serum by high-performance liquid chromatography with fluorimetric
E Bousquet1, S Tirendi, N A Santagati
1Istituto di Chimica Farmaceutica e Tossicologica, Facoltá di Farmacia, Università di Catania, Italy.
Abstract:
A method is reported for the determination of the anticonvulsant sodium valproate in serum by fluorimetric detection. HPLC is performed on a reversed-phase column with a mobile phase of acetonitrile/water (65:35, v/v) with cyclohexane carboxylic acid as an internal standard. Samples were derivatized with 4-bromomethyl-7-methoxycoumarin and the optimized conditions are reported.
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