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Related Experiment Videos

Comparison of two simple methods for determining malaria parasite density.

B M Greenwood1, J R Armstrong

  • 1Medical Research Council Laboratories, Fajara, Banjul, The Gambia.

Transactions of the Royal Society of Tropical Medicine and Hygiene
|March 1, 1991
PubMed
Summary

Comparing malaria parasite density methods, counting parasites per high power field on a thick blood film proved more accurate than counting parasites per white blood cell. This improves malaria diagnosis accuracy.

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Area of Science:

  • Medical Parasitology
  • Diagnostic Microbiology

Background:

  • Accurate malaria parasite density is crucial for effective treatment and prognosis.
  • Traditional methods often require complex procedures or specialized equipment.

Purpose of the Study:

  • To compare the accuracy of two simple thick blood film methods for malaria parasite density determination against a standard thin film method.
  • To identify a more reliable and accessible method for quantifying malaria parasites.

Main Methods:

  • Method 1: Parasite density calculated by multiplying parasites per white blood cell by an average white blood cell count (8000/µL).
  • Method 2: Parasite density calculated from parasites per high power field and assumed blood volume per field.
  • Comparison against a gold standard method involving red blood cell count and thin blood film analysis.

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Main Results:

  • The method using parasites counted per high power field demonstrated higher accuracy compared to the parasite/white blood cell ratio method.
  • Variability in blood volume on thick films was likely less than white blood cell count variability in the study population, explaining the accuracy difference.

Conclusions:

  • Counting malaria parasites per high power field on a thick blood film is a more accurate and simpler alternative for density determination.
  • This method offers improved diagnostic capabilities in resource-limited settings for malaria management.