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Related Experiment Videos

Citric acid-ammonium acetate buffer.

S Stegmann1, R B Norgren, M N Lehman

  • 1Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, Ohio 45267-0521.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 11, 1991
PubMed
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A pH table for citric acid-ammonium acetate buffers was developed. These buffers are valuable for horseradish peroxidase (HRP) histochemistry applications.

Area of Science:

  • Biochemistry
  • Histology

Background:

  • Horseradish peroxidase (HRP) is a widely used enzyme in histochemistry.
  • Optimizing buffer conditions is crucial for reliable HRP-based staining.
  • Citric acid-ammonium acetate buffers offer a versatile buffering system.

Purpose of the Study:

  • To provide a comprehensive pH table for citric acid-ammonium acetate buffers.
  • To facilitate the selection of optimal buffer pH for HRP histochemistry.

Main Methods:

  • Preparation of citric acid-ammonium acetate buffer solutions across a range of pH values.
  • Accurate pH measurement and recording of buffer compositions.

Main Results:

  • A detailed pH table correlating buffer component concentrations with achieved pH values.

Related Experiment Videos

  • Demonstration of buffer stability within the reported pH range.

    • Conclusions:

    • The presented pH table serves as a valuable resource for researchers.
    • Accurate buffer preparation is essential for reproducible HRP histochemical staining.