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A hemolysin associated with the mumps virus

H R MORGAN, J F ENDERS, P F WAGLEY

    The Journal of Experimental Medicine
    |November 1, 1948
    PubMed

    Insights

    A novel hemolysin produced by mumps virus in chick embryos was identified. This mumps hemolysin, distinct from influenza virus, shows specific inhibitory responses and temperature/pH activity.

    Area of Science:

    • Virology
    • Immunology
    • Biochemistry

    Background:

    • Mumps virus infection can lead to various complications.
    • Understanding virus-host interactions is crucial for disease management.

    Purpose of the Study:

    • To identify and characterize a hemolysin produced by mumps virus in chick embryos.
    • To investigate the properties and potential role of this hemolysin in mumps pathogenesis.

    Main Methods:

    • Infection of chick embryos with mumps virus and collection of amniotic/allantoic fluids.
    • Hemolysis assays using erythrocytes from different species (human, chicken, sheep).
    • Temperature, pH stability, and serum inhibition studies.
    • Adsorption-elution experiments with red blood cells.

    Main Results:

    • A hemolysin was detected in fluids from mumps-infected chick embryos, but not in normal fluids or those infected with influenza virus strains.
    • The hemolysin was inhibited by convalescent human and monkey serum, with less effect from acute phase serum.
    • Optimal activity was observed at 37°C and pH 7.0-8.0, with inactivation at 50°C and 4°C.
    • Adsorption and elution of the hemolysin altered red blood cell osmotic fragility, unlike influenza virus treatment.

    Conclusions:

    • Mumps virus produces a specific hemolysin in chick embryos with distinct biochemical properties.
    • This hemolysin may play a role in mumps pathogenesis and exhibits enzyme-like characteristics.
    • Further research is needed to elucidate the precise relationship between the hemolysin and hemagglutinin.
    Keywords:
    MUMPS/virus

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