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A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis
M F De Bolle1, I J Goderis, F R Terras
1F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Electrophoresis
|June 1, 1991
Abstract:
A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. alpha-purothionin, Urtica dioica agglutinin, and tobacco chitinase.