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Immunity conferred by Aro- Salmonella live vaccines
C E Hormaeche1, H S Joysey, L Desilva
1Department of Pathology, University of Cambridge, U.K.
Microbial Pathogenesis
|February 1, 1991
Summary
Salmonella aroA vaccines provide protection against homologous strains, but cross-protection varies. Protection specificity is linked to the Salmonella parent strain background, not just LPS antigens, indicating other antigens are involved.
Area of Science:
- Immunology
- Microbiology
- Vaccinology
Background:
- Salmonella aroA mutants are attenuated vaccines.
- Understanding vaccine-induced protection specificity is crucial for effective Salmonella control.
Purpose of the Study:
- To investigate the specificity of protection conferred by aroA-Salmonella vaccines.
- To determine the role of LPS antigens and parent strain background in vaccine efficacy.
Main Methods:
- BALB/c mice were vaccinated intravenously with aroA-Salmonella strains (S. typhimurium, S. enteritidis, S. dublin).
- Mice were challenged with homologous or heterologous wild-type Salmonella strains with varying LPS types.
- Protection was assessed by survival rates and lethal dose 50 (LD50) values.
Main Results:
- S. typhimurium aroA provided strong protection against S. typhimurium but not S. enteritidis.
- Protection specificity was more influenced by the Salmonella parent strain (S. typhimurium vs. S. enteritidis) than by O-antigen type (O-4 vs. O-9).
- S. enteritidis and S. dublin aroA vaccines showed good homologous protection, but efficacy waned over time.
Conclusions:
- AroA-Salmonella vaccine efficacy is strain-specific and influenced by factors beyond LPS antigens.
- The parent strain background plays a significant role in determining protective immunity.
- Further research is needed to identify the key protective antigens in aroA-Salmonella vaccines.